User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/09/20

UNAM Genomics Mexico 2011

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Parts assembly ABSTRACT PCR to amplify HydEF1 I did two PCR reactions in order to see which annealing temperature is best to amplify HydEF1. I also change some reagents such as the Buffer for the polymerase. I use the following proportions: H2O 22.8μL Buffer GC 10μL dATP 1 μL dGTP 1 μL dTTP 1 μL dCTP 1 μL Primer Fwd 5 μL Primer Rev 5 μL DNA template 1.2 μL DMSO 1.5 μL Phusion Pol 0.5 μL Cycles Gel Lanes Gel 95°C 5min 98°C 10s 30Cy 60°C-62°C 30S 30Cy 72°C 2 min 30Cy 72°C 10min 4°C Lane1 Ladder Lane2 HydEF1 60°C Lane3 HydEF1 62°C