User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/09/16

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Parts assembly


  • PCR of HydEF1 using a primer for Preffix.

  • In order to see what is wrong with HydEF (because we can´t obtain the product) I performed a PCR using a primer that binds to the preffix and the primer HydEF1.Int.Rev. With the following proportions:
H2O 22.8μL
Buffer HF 10μL
dATP 1 μL
dGTP 1 μL
dTTP 1 μL
dCTP 1 μL
Primer Fwd 5 μL
Primer Rev 5 μL
DNA template 1.2 μL
DMSO 1.5 μL
Phusion Pol 0.5 μL
  • Fibo run an electrophoresis gel with the reactions but no bands where observed.