User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/08/22

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Competent cells


  • Planning to make competent cells.

  • The synthesis hasn´t arrived yet so I continue doing incidental things for the whole team. Today we noticed a shortage in competent cells, this are very important for the validation of some experiments such as ligations or for transform plasmid of interest. So today I checked the protocol and I ask some help from my advisors and made a plan to do competent cells.
  • It is a protocol for 3 days, so I divide the work. Vlad accept to help me, so we are going to work together. Tonight he is going to put 5 mL liquid cultures inoculated with E. coli DH5α and left them incubating at 37°C, 200 rpm overnight.