User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/07/29

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Plasmid Extraction

  • Extraction of pBBRMCS5 standard using new solutions.

Lanes:

1. Isolation 15
2. Isolation 2
3. Isolation 3
4. Isolation 4
5. Isolation 5
6. Isolation 5
7. Isolation 7
8. Isolation 1
9. Isolation 8
10. Isolation 9
11. Isolation 10
12. Isolation 11
13. Isolation 12
14. Isolation 13
15. Isolation 14

Pae 29 jul 2011.JPG

As you see, the solutions didn´t work well, we have to extract more plasmid again.


  • Because the solutions we have in the lab didn´t work well, Pablo did new ones using the protocol [[User:Pablo_E._Garcia_Nieto| 4. Preparación de reactivos para extracción de DNA plasmídico. He left 15 tubes with E. coli cultures growing overnight, so today I isolate the plasmid pBBRMCS5 using the following protocol: Plasmid extraction.
  • Pablo ran an electrophoresis gel to see the integrity of the isolated plasmid:

Lanes:

1. Isolation 15
2. Isolation 2
3. Isolation 3
4. Isolation 4
5. Isolation 5
6. Isolation 5
7. Isolation 7
8. Isolation 1
9. Isolation 8
10. Isolation 9
11. Isolation 10
12. Isolation 11
13. Isolation 12
14. Isolation 13
15. Isolation 14

Pae 29 jul 2011.JPG

As you see, the solutions didn´t work well, we have to extract more plasmid again. |}