User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/07/12

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Digestion continuation

  • Today I made an electrophoresis gel with the digestions I did yesterday. This is the result:

Lanes:

 1)Ladder
 2)Non digested plasmid
 3)Plasmid digested with XbaI
 4)Plasmid digested with PstI

Pau 12 jul 2011.JPG

As you see both enzymes cut correctly the plasmid, so we assure that the plasmid has the corresponding prefix and suffix regions so it incorporated the RFP.