User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/07/07
|UNAM Genomics Mexico 2011||Main project page|
Previous entry Next entry
Standard plasmid isolation
In this dish we only observe one red colony so we take some bacteria from that line and we put them in both liquid medium and solid medium. The red colony suggests that one ligation of the plasmid with the standard PCR was correct.
1. Ladder 10 kb 2. Pablo´s isolation 1. 3. Pablo´s isolation 2. 4. Paulina's isolation 1. 5. Pauilna's isolation 2.
The isolation in the third lane doesn´t go well. The next step is to digest with EcoR1 and Pst1 in order to see if the region with the preffix and suffix was correctly added to the plasmid.