User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/06/22

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E. coli BL21_DE3 re-culture


  • Culture in liquid LB medium of BL21_DE3 cells.

  • Today approximately at 10:30 pm I made 4 cultures (and one control tube) in liquid LB medium of BL21_DE3 cells that were previously transformed with pBBRMCS5 plasmid. The idea of this was to transfer the plasmid to a DCM- cell to make the proper digestion. The cultures were made in 1 ml of LB medium and I left one tube for control. Tomorrow morning we are going to extract pBBRMCS5 plasmid.