TAP medium preparation
ABSTRACT
- Completion of TAP media preparation. Making of phosphate buffer; filtration of Trace Elements solution and preparation of 500ml of both liquid and solid medium.
- Today Pablo and I finished the preparation of the TAP media for Chlamydomonas reinhardtii.
- We filter the Trace elements solution with a 50 ml syringe and filters of 22μm.
- We prepare 0.25 Lt of phosphate buffer 1M pH 7, with this proportions:
| K2HPO4
|
43.54
|
| KH2PO4
|
20.97
|
- Finally we mix the following proportions to make 500 ml of medium:
| Beijerincks 20X
|
25 ml
|
| Acetic acid
|
500 μl
|
| Tris
|
1.21 g
|
| Phosphate buffer 1M pH 7
|
500 μl
|
| Trace elements solution
|
500 μl
|
We prepare 500 ml of liquid medium and 500 ml of solid medium by adding 8g of agarose.
This same day we re-culture Chlamydomonas in 2 petri dishes with solid medium and in 2 tubes with liquid medium. We leave 2 controls also.
|
UNAM Genomics Mexico 2011
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