User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/02/04

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Qualitative Analysis of Protease Activity

Objective

The reactions will be analyzed qualitatively for protease activity.

Procedure

UV-VIS was run from 200-800 nm, with a large-volume quartz cuvette. The solutions were mixed before being added. The cuvette was washed twice with 0.5 M HCl, once with 1% SDS, and dried with Methanol.

Results

Pepsin

Pepsin Qual Feb 4.png

Trypsin

Trypsin Qual Feb 4.png

Chymotrypsin

Chymotrypsin Qual Feb 4.png

Proteinase K

Proteinase K Qual Feb 4.png

Analysis

Trypsin, Chymotrypsin, and Proteinase K all had discernible degradation of AuNP fibers at the conditions tested. The absorbance values were all relatively similar between the proteases. The maximum wavelengths were between 526 and 529 nm, which were smaller than expected. This means the colloid produced had smaller diameter particles. This will be confirmed with gel analysis.