User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/02/04

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Qualitative Analysis of Protease Activity


The reactions will be analyzed qualitatively for protease activity.


UV-VIS was run from 200-800 nm, with a large-volume quartz cuvette. The solutions were mixed before being added. The cuvette was washed twice with 0.5 M HCl, once with 1% SDS, and dried with Methanol.



Pepsin Qual Feb 4.png


Trypsin Qual Feb 4.png


Chymotrypsin Qual Feb 4.png

Proteinase K

Proteinase K Qual Feb 4.png


Trypsin, Chymotrypsin, and Proteinase K all had discernible degradation of AuNP fibers at the conditions tested. The absorbance values were all relatively similar between the proteases. The maximum wavelengths were between 526 and 529 nm, which were smaller than expected. This means the colloid produced had smaller diameter particles. This will be confirmed with gel analysis.