User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/02/04

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Qualitative Analysis of Protease Activity Objective The reactions will be analyzed qualitatively for protease activity. Procedure UV-VIS was run from 200-800 nm, with a large-volume quartz cuvette. The solutions were mixed before being added. The cuvette was washed twice with 0.5 M HCl, once with 1% SDS, and dried with Methanol. Results Pepsin Trypsin Chymotrypsin Proteinase K Analysis Trypsin, Chymotrypsin, and Proteinase K all had discernible degradation of AuNP fibers at the conditions tested. The absorbance values were all relatively similar between the proteases. The maximum wavelengths were between 526 and 529 nm, which were smaller than expected. This means the colloid produced had smaller diameter particles. This will be confirmed with gel analysis.