User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/11/11

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Calibration Curves

Bradford

I remade a calibration curve with 0.12 g/L Lysozyme. The final concentrations in each sample are below:

Volume of Stock Final Concentration
20 µL 2.4 µg/mL
40 µL 4.8 µg/mL
60 µL 7.2 µg/mL
80 µL 9.6 µg/mL
100 µL 12 µg/mL

Results

11.11.14 Bradford Calibration Spectra MGPR.png

11.11.14 Bradford Calibration Curve MGPR.png


CaCl2 Calibration Curve

I remade a CaCl2 calibration curve using the Ca2+ ISE probe.

Concentration (mM) Potential (mV)
0.005 (-17.4)
0.05 3.3
0.5 33.5
5 59.6
15 71.8
25 78.7
35 82.7
45 85.2
50 84.1


CaCl2 Calibration Curve.png