User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/10/28

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Continuation of Lysozyme vs KI Dialysis I used the I- ISE probe to rerun concentration of I- in the dialysis solutions. Results Sample Potential (mV) ln[KI] [KI] (mM) L-50 mM KI -253.7 3.014259251 20.37399334 L-25 mM KI -237.7 2.324990307 10.22658096 L-10 mM KI -215.8 1.38155344 3.981081197 L-5 mM KI -199.9 0.696592427 2.006902376 L-2 mM KI -177.4 -0.272692026 0.761327221 25 mM KI -240.1 2.428380649 11.34050295 10 mM KI -210.8 1.166156895 3.209633945 5 mM KI -196.2 0.537198983 1.711207024 2 mM KI -173.7 -0.432085469 0.649153892 Analysis of Lysozyme vs CaCl2 Dialysis Monika and I reran this dialysis because the lysozyme crashed out. We ran Bradford, UV, Fluorescence, and Ca2+ ISE. Results Fluorescence: Bradford: UV-VIS: NB - I had to rerun the 5 µM sample because the wavelength range was not correct on the first run on Wednesday. I also reran a stock and blank for comparison.