User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/10/28

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Continuation of Lysozyme vs KI Dialysis

I used the I- ISE probe to rerun concentration of I- in the dialysis solutions.

Results

Sample Potential (mV) ln[KI] [KI] (mM)
L-50 mM KI -253.7 3.014259251 20.37399334
L-25 mM KI -237.7 2.324990307 10.22658096
L-10 mM KI -215.8 1.38155344 3.981081197
L-5 mM KI -199.9 0.696592427 2.006902376
L-2 mM KI -177.4 -0.272692026 0.761327221
25 mM KI -240.1 2.428380649 11.34050295
10 mM KI -210.8 1.166156895 3.209633945
5 mM KI -196.2 0.537198983 1.711207024
2 mM KI -173.7 -0.432085469 0.649153892

Analysis of Lysozyme vs CaCl2 Dialysis

Monika and I reran this dialysis because the lysozyme crashed out. We ran Bradford, UV, Fluorescence, and Ca2+ ISE.

Results


Fluorescence:

10 28 Lysozyme CaCl2 Fluorescence Chart.png

Bradford:

Lysozyme CaCl2 Bradford 3500MW 10 28 Chart.png

UV-VIS:

Lysozyme CaCl2 UV 10 29 Chart.png

NB - I had to rerun the 5 µM sample because the wavelength range was not correct on the first run on Wednesday. I also reran a stock and blank for comparison.