User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/10/08

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Analysis of Dialysis

There were four tasks completed:

  1. Fluorescence/UV-VIS
  2. Ca2+ ISE
  3. pH
  4. Make new colloid solution


Fluorescence and UV-VIS

Fluorescence was run on both the 3500 MW and 20k MW dialyses.

3500 MW Dialysis

3500MW Dialysis 1in100 NoBradford.png

UV VIS Dialysis 10 8 Rerun Chart.png

Lysozyme 20k Dialysis Fluorescence Emission Chart.png


20k MW Dialysis

Bradford Dialysis 20k Analysis 10 8.png

Lysozyme 20k Dialysis Fluorescence Correct Emission Chart.png

Solution Absorbance Concentration of Protein (µg/mL)
5uM Lysozyme 0.02 -3.733009709
5uM Lysozyme Soak 0.022 -3.63592233
50 mM Colloid 0.012 -4.121359223
50 mM Colloid Soak 0.01 -4.218446602
50 mM Lysozyme 0.035 -3.004854369
50 mM Lysozyme Soak 0.025 -3.490291262
50 uM Colloid 0.013 -4.072815534
50 uM Colloid Soak 0.023 -3.587378641
50 uM Lysozyme 0.018 -3.830097087
50 uM Lysozyme Soak 0.028 -3.344660194

Ca2+ ISE

Ca2+ ISE was run on the 2 solutions from the 3500 MW dialysis. Here are the results:

Concentration mV
500 µM CaCl2 34.3 mV
L-500 µM CaCl2 31.1 mV
50 mM CaCl2 77.5 mV
L-50 mM CaCl2 77.0 mV

pH

pH was taken on the 3500 MW dialysis solutions containing H+:

  1. HCl-Lysozyme - 7.926
  2. HCl Soak - 8.138

Colloid synthesis

New 30:1 colloid was synthesized by Monika. She'll have the results.

Titrations

Monika ran a KI precipitation titration to determine concentration of I-. She has the results here.