User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/09/17

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pH and Conductivity Calibration of NaCl and Glycine Buffers

Yesterday and today, we ran pH and conductivity calibrations for 50 mM NaCl and 50 mM Glycine buffers (pH 3.5, 0.4 mL 1M HCl used). A 2x, 5z, 10x, 20x, 50x, 100x, 200x, and 500x dilution of each was prepared for use.

NaCl Glycine Tables 17 Sept.png

The results are as follows:

NaCl

NaCl Conductivity 17 Sept.png

NaCl Log Conc pH 17 Sept.png

NaCl pH 17 Sept.png

Glycine

Glycine Conductivity 17 Sept.png

Glycine Log Conc pH 17 Sept.png

Glycine pH 17 Sept.png

Lysozyme 1g/L Data

pH and conductivity were taken for the stock solution and for a 1 in 6 dilution.

  1. Stock
    1. pH - 5.700
    2. Conductivity - 144.6 µS/cm
  2. 1 in 6 Dilution
    1. pH - 6.123
    2. Conductivity - 24.14 µS/cm

Dialysis

We used the two buffers to run dialysis of 1 g/L lysozyme. We used 3500 and 25k MW dialysis tubing. The 3500 was used for NaCl, Glycine, and H2O, and colloid solution (made on 26 August). These were left overnight to today.