User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/09/10
|Project name||Main project page|
Previous entry Next entry
Bradford Assay for BSA and Lysozyme
Today, we ran a Bradford Assay to determine actual protein concentration in solutions of BSA and lysozyme.
Stock solutions of 0.9% w/v NaCl and 50 mM Tris/50 mM NaCl were prepared. 1 mg/mL solutions of BSA and lysozyme were prepared with the saline in a total volume of 5 mL. Bradford reagent was prepared in a 1 in 4 dilution. A range of dilutions was prepared, at 1, 2, 5, 10, 15, and 20 µg/mL protein, 200 µL Bradford reagent, and Tris/NaCl buffer to bring the volume to 1 mL total.
Beer's Law plots were constructed with the data.