User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/31

Project name

FLP

Host: Saccharomyces cerevisiae
Classification: tyrosine recombinase
structure
- Length
- Amino-Acid-Sequence
- Domains
  - six tyrosine kinase conserved residues: R191, K223, H305, R308, W330, Y343 (nucleophilic tyrosine and catalytic pentad)
Target sequence: two identical simple sequence < 50 bp
Mechanism:
Engineering
- Mutation
  - Mutation could change specificity to HK022's site
- Chimeric
- Directed-Evolution
Exision:
- Unnatural-Host