User:Pakpoom Subsoontorn/Notebook/Genetically Encoded Memory/2008/10/30

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GammaDelta

  • Host: Escherichia coli
  • Classification: serine resolvase
  • structure:
    • Length: 183 aa
    • Amino-Acid-Sequence
    • Domains
      • Catalytic site has R8, S10, D67, R68
      • Li et al 2005 science
  • Target sequence: two identical simple sequence < 50 bp
    • res-I, res-II, res-III
  • Mechanism:
    • two dimer per site (see diagram from the ref)
  • Engineering
    • Mutation
      • Mutation could change specificity to HK022's site
    • Chimeric
    • Directed-Evolution
  • Exision:
    • Unnatural-Host