User:Omar Choudary/Notebook/Omar Choudary CHEM-571 AU-2011/2012/2012/02/08
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Au/BSA (70) Ratio:
.42 mL HAuCl4 + 1 mL BSA + 8.5 mL H2O
Au/BSA (166) Ratio:
1 mL HAuCl4 + 1 mL BSA + 8 mL H2O
1.514 g Tris + 3.51 mL 0.948M HCl and then filled to 250 mL with H2O.
pH = 8.73
The tris refolding buffer will be used next week to raise the pH of the solutions after the AuNP synthesis to the 8 to 9 pH range. The higher pH is a mandatory step for tagging the lysine residues in the BSA with the dye which need to be deprotonated (pKa = 8.95 at the amino group). The BSA control was made with the same amount of acid but with HCl instead of HAuCl4; so the solution is identical to the AuNPs except the Gold is absent.