User:Omar Choudary/Notebook/Omar Choudary CHEM-571 AU-2011/2012/2011/12/07

From OpenWetWare
Jump to: navigation, search
BDLlogo notext lr.png Chem-571 Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


Objective

  • Try to obtain a purple solution with a 166 ratio and protein fibers with 70 ratio.
  • Reproduce 12/6 experiment (with 166 ratio) to yield purple solution.
  • Reduce the total volume of the 70 ratio solution and see if we obtain purple protein fibers.

Description

  • 70 molar ratio (Au/BSA):

0.05 mL of HAuCl4 (4.17mM)

0.1 mL BSA (17.7µM)

0.2 mL water

Placed in heatblock for 2 hours (constant temperature).


  • 166 molar ratio (Au/BSA):

0.1 mL of HAuCl4 (2.9mM)

0.1 mL of BSA (17.7µM)

0.8 mL of water

Placed in heatblock for 1 hours and then put on ice.

Data

  • Both the 12/7 and 12/6 experiments yielded a homogeneous purple solution. This was the expected result, indicating that a valid method of forming purple solution at a 166 Au/BSA molar ratio had been developed.
  • The 70 Au/BSA molar ratio yielded a purple solution meaning that a method to form purple fibers at a 70 Au/BSA molar ratio has not been developed.

OC-12-7-1.jpg

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.