User:Omar Choudary/Notebook/Omar Choudary CHEM-571 AU-2011/2012/2011/11/02
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Objective
Description
1. Mixture created in the following order: 1 mL BSA (15.5 μM), 1 mL HAuCl4 (2.9mM), 8 mL H2O. 2. Placed in oven, removed every 30 minutes for 10 minutes over the course of 2 hours.
1. LB/Agar Plate A. Mixed 0.875g LB, 0.7g Agar, 30 mL H2O B. Autoclaved for 90 min C. Added 35 μL Ampicillin (Antibiotic), 35 mL H2O D. Poured into petri dish until bottom of dish is fully covered E. Solution left to dry until solid in petri dish
A. Placed DNA solution from PCR on ice B. Added 5 μL DNA solution to 30 μL of E.Coli cells C. Incubated cells on ice for 30 min D. Moved cells to heat block set at 42 degrees for 30 seconds (Heat Shock) E. Cells then returned to ice for 5 min F. 250 μL SOC media added to cells G. Incubated in shaker set at 37 degrees celsius for one hour Data
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