User:Omar Choudary/Notebook/Omar Choudary CHEM-571 AU-2011/2012/2011/10/26
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1. Remove Coomassie Blue stain
2. Add destaining solution
3. Leave on shaker
1. Tube 1: 1 mL BSA (15 μM) , 2 mL HAuCl4 (0.75 mM), 7 mL H2O
2. Tube 2: 1 mL BSA (15 μM) , 9 mL H2O
3. Tubes placed in oven set at 80 degrees Celsius, and removed for 10 minutes every 30 minutes over the course of 2 hours.
Tube 1 was a clear solution, with a gray and green protein fiber aggregate.
Tube 2 remained clear.
Results indicate that while the protein of interest was isolated, it was not pure enough for further experimentation.