User:Omar Choudary/Notebook/Omar Choudary CHEM-571 AU-2011/2012/2011/10/18

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  • Create cysteine labeled Fluorophore using Fluorophore 5-(Iodoacetamido) flourescein (515.3 g/mol) and cysteine (121.6 g/mol).


  • We're going to use 5X more 5-(Iodoacetamido)fluorescein than cysteine.
  • Measured 1 mg of 5-(Iodoacetamido)fluorescein. (yellow powder)
  • Added 1 µL of cysteine stock solution.
  • Added Tris buffer (pH 9.3) until fluorescein is fully dissolved. (1ml)
  • Vortexd solution.
  • Added 100µL of DTT after 2 hours of reaction.