User:Omar Choudary/Notebook/Omar Choudary CHEM-571 AU-2011/2012/2011/09/28

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Objective

  • Perform gold nanoparticle (AuNP) synthesis experiment using Chloroauric Acid (HAuCl4), Bovine Serum Albumin (BSA), and Water.
  • Check if DNA transformation experiment performed on 9/27 was successful.

Description

  • Mixed 0.581 mL HAuCl4 (.25mM) with .974 mL BSA (15μM) and 8.45 H2O Buffer in test tube.
  • UV-Vis was set at 80 Degrees Celsius, and spectra (200 - 800 nm) was taken every 30 mins for 2 hours.
  • After 2 hours the test tube was removed from the oven, wrapped in aluminum foil, and stored at room temperature.

Data

  • Full spectrum of mixture. When taking the spectra at 150 mins, the UV-Vis compartment was accidentally opened.

OC-9-28-1.jpg


  • Spectrum focused at 550 nm.

OC-9-28-2.jpg


  • Change in absorbance at 550 nm over the course of 150 min.

OC-9-28-3.jpg


  • Homogeneous Purple Solution


OC-9-28-4.jpg

Notes

  • The molar ratio of Au/BSA for the mixture was 9.92.
  • The mixture was a homogeneous purple solution.
  • The trendline on the graph of Absorbance vs. Time at 550 nm is increasing indicating AuNP synthesis.
  • All spectra were taking using Shimadzu-2550 spectrophotometer.