- Synthesize Gold Nanoparticles(AuNP's) using Bovine Serum Albumin (BSA) and Chloroauric Acid (HAuCl4) in water.
- Created 10mL aqueous mixture of 0.0015 mM BSA and 0.25 mM HAuCl4 using water at room temperature.
- Placed mixture into oven set to 80 degrees Celsius.
- Using UV-Vis Spectrophotometer obtain UV-visible spectra (200 - 900nm) of mixture every 30 minutes for 2 hours.
- Theoretical Measurements:
1.5*10^-6 moles BSA / 1 L * .01 L * 66,776 g BSA / 1 Mole BSA = .001 g BSA
2.5*10^-5 moles HAuCl4 / 1 L * .01 L * 339.785 g HAuCl4 / 1 Mole HAuCl4 = 8.49*10^-5 g HAuCl4
.001 g BSA
.006 g HAuCl4
- Entire UV-Vis spectrum of mixture.
- UV-Vis spectrum focused at 550 nm to observe change in absorbance.
- Change in absorbance of mixture only at 550 nm. The increasing trend-line indicates AuNP synthesis is occurring.
- The molar ratio of Au/BSA was 1176.
- The mixture was a clear solution.
- The mixture was wrapped in aluminum foil after being removed from the oven.
- Distilled water was used as the buffer in this experiment. A blank using just the distilled water was measured first and then BSA/HAuCl4 mixture was initially measured and then placed into the oven. The data projected on both graphs had been corrected using the blank.
- All UV-Vis spectra were taken using a Shimadzu UV-2550.
- The quartz cuvette used to obtain UV-Vis spectra had purple staining that could not be removed by washing.