User:Nouf Abuladel/Notebook/CHEM-471/2015/09/23

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Objective

  • To learn about Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC) and X-ray Powder Diffraction (XRD). Additionally, for this lab we'll be working on those devices to get spectra for the lysozyme AuNP fibers (using the 5 mL) fiber samples that we have prepared previously.

Description

  • Before preparing the appropriate samples for each device, all 10 tubes of fibers were transferred into 5 falcon tubes. The fibers were centrifuged for 5 mins at 4000 R. These centrifuged fibers were used to prepare samples for each device.

- 1st: Sample prepration for XRD:

  • Drops of fibers were added into the appropriate amount of PlayDo and mixed together. The fiber-PlayDo sample was placed into the special lid for XRD.

- 2nd: Sample preparation for DSC:

  • The masses of 4 lids and pans were recorded.
  • 2 lids and pans were used for the fiber and the other two were used for lysozyme.
  • For fiber:

Sample 1

  1. lid and pan mass = 51.37 mg
  2. sample mass = 3.49 mg
  3. sample+lid+pan mass = 53.73 mg

Sample 2

  1. lid and pan mass = 50.80 mg
  2. sample mass = 4.43 mg
  3. sample+lid+pan mass = 53.74 mg
  • For lysozyme:

Sample 3

  1. lid and pan mass = 51.237 mg
  2. sample mass = 3.04 mg
  3. sample+lid+pan mass = 54.38 mg

Sample 4

  1. lid and pan mass = 50.71 mg
  2. sample mass = 3.63 mg
  3. sample+lid+pan mass = 54.44 mg
  • The four samples were pressed three times using a pressor.
  • Sample 1 was placed in well #27 in the DSC. The samples 2, 3 and 4 were placed in wells 28, 29 and 30.
  • The cycling procedure:
  1. Equilibrate at 20°C
  2. Ramp up to 5°C/min and to 100°C.
  3. Isothermal - 3 mins
  4. Ramp down to 5°C/min and to 20°C
  5. Repeat (blue arrow)
  6. Lastly, external event (OFF)

Data Analysis

Observations

This area is for any observations or conclusions that you would like to note.


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