User:Nouf Abuladel/Notebook/CHEM-471/2015/09/09

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Objective

  • To determine the molar absorptivity of lysozyme. We will use this information as we move on to quantifying protein concentration with the Bradford assay. Also, to determine how protein concentration affects fluorescence intensity.

Procedure

Screen Shot 201DDD5-09-15 at 2.48.44 PM.png

Data Analysis

tubes intial mass Thermolysin mass Mg grams moles concemtration
1.03104 0.8 0.0008 2.31214E-08 2.31214E-05
1.02859 1.33 0.00133 3.84393E-08 3.84393E-05
1.0199 1.29 0.00129 3.72832E-08 3.72832E-05
1.02753 1.21 0.00121 3.49711E-08 3.49711E-05
1.01646 1.43 0.00143 4.13295E-08 4.13295E-05
1.03078 1.12 0.00112 3.23699E-08 3.23699E-05
1.02419 1.21 0.00121 3.49711E-08 3.49711E-05
1.01308 1.29 0.00129 3.72832E-08 3.72832E-05
1.02896 1.04 0.00104 3.00578E-08 3.00578E-05
1.0288 1.29 0.00129 3.72832E-08 3.72832E-05
1.02602 1.08 0.00108 3.12139E-08 3.12139E-05
1.02456 1.03 0.00103 2.97688E-08 2.97688E-05
1.01719 0.75 0.00075 2.16763E-08 2.16763E-05
1.02863 1.24 0.00124 3.58382E-08 3.58382E-05
1.0285 1.18 0.00118 3.4104E-08 3.4104E-05

Observations

500 pxl


500 pxl

Matt Hartings You need to add your UV Vis and fluorescence data