User:Noah Benjamin/Notebook/Experimental Biological Chem/2011/11/15
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Cells were sonicated (30 sec ON/30 sec OFF 3 times) Solutions were made: 1 L 50 mM Tris + 1 M NaCl pH 9, filtered 1 L 50 mM Tris pH 9, filtered 4L 50 mM Tris pH 9 4L 50 mM Tris pH 9 cells were placed in dialysis tubes and in 50 mM Tris overnight in a -80 C.
green A: 250µL Au (4.17 mM) + 500µL BSA (30µM) + 9.25mL water green B: 500µL An (4.17 mM) + 1000µL BSA (30µM) + 8.50mL water Left in oven the entire time.
red A: 500µL Au (4.17 mM) + 500µL BSA (30µM) + 9 mL water red B: 1000µL Au (4.17mM) + 1000µL BSA (30µM) + 8mL water In 50min, Out 10min of the oven.
Both "Green" tubes produced purple solution, both "Red" tubes produced aggregate purple fibers.
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