User:Noah Benjamin/Notebook/Experimental Biological Chem/2011/11/02
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Testing "New Gold" to check if the gold was the problem, not our methods
1mL Au (2.5mM) 1mL BSA (15µM) 8mL water Left in oven 50min, taken out for 10 min, repeat.
DPN1 was added to to the PCR products to "chop the non PCR DNA" 10 µL of the DNA was added to a separate tube 2 µL of loading buffer was added to the tube
The solution was then loaded into the gel and was run
LB Agar Plates:
.875 g LB .7 g Agar 35 mL distilled water
The cells were then plated on LB/agar plates with a 35 μL of antiobiotics 30 μL of competent cells 5 μL of DNA were combined and incubated on ice for 30 minutes The DNA/cell mixture was then heat shocked at 42C for 30 sec incubated on ice for 5 min 250μL of SOC media was added to the mixture incubated in the shaker at 37C for 1 hr 100μL of cells were spread (using sterile techniques) on the LB/agar plate The plate was then inverted and stored in a 37C oven overnight
The new gold successfully produced purple fiber aggregation, instead of gray. It was the gold causing the discoloration.
This area is for any observations or conclusions that you would like to note.