User:Noah Benjamin/Notebook/Experimental Biological Chem/2011/09/07
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An adjusted repetition of the experiment from 8/31/11.
1 mL of 2.5 mM stock solution of chloroauric acid and 1 mL of 15uM BSA stock solution BSA were combined with 8 mL of 7.55 pH Tris buffer in a test tube such that the solution totaled approximately 10 mL and was 0.25 mM chloroauric acid and 1.5 uM BSA. This was repeated in another test tube using distilled water instead of Tris buffer. The solutions were then analyzed in the UV Spectrometer as follows: First controls of only Tris buffer/ distilled water were analyzed. Samples of the solutions were then analyzed (labeled time 0). The solutions were then placed in an oven at 80 degrees Celsius and samples were analyzed at intervals of 30 minutes until there was no longer time left to conduct more analyses.
Note: Actual volumes of Tris buffer and distilled water used were 8.15mL and 8.10mL respectively.
Noah, what do you make of your observations and data here? What did you actually observe being formed in the experiment? Along with what the UV-Vis shows ... what do your eyes say happened? Matt Hartings 11:50, 14 September 2011 (EDT)
Any changes in the absorption at t2 are changes in the baseline, not spikes at that particular wavelength. This means there was no significant data observed that indicates something unique.
During the experiment, purple stringy material formed in the solution. This may have affected t2 if a piece of the purple material were in the samples analyzed.