User:Nicole Bonan/Notebook/Quantum Dots/2014/03/21

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Objective

Stock solutions of the following were synthesized in order to provide a more stable buffer system that the quantum dots would stay dissolved in:

  1. 50mmol phosphate and 100mmol sodium chloride, pH=7
  2. 50mmol acetate, pH=5
  3. 50mmol phosphate, pH=7
  4. 50mmol tris, pH=9
  5. 50mmol aceate and 100mmol sodium chloride, pH=5
  6. 50mmol tris and 100mmol sodium chloride, pH=9

The pH of the stock solutions was adjusted by adding either hydrochloric acid or sodium hydroxide drop wise. The stock solutions were labeled 1-6. After centrifugation and decantation of the quantum dot solutions, the stock solutions were added to the quantum dots.

Data

Solution 1: 50mmol phosphate and 100mmol sodium chloride, pH=7

  • 0.710g phosphate
  • 0.584g sodium chloride
  • 100mL distilled water
  • 1200µL hydrochloric acid

Solution 2: 50mmol acetate, pH=5

  • 0.680g acetate
  • 100mL distilled water
  • 1300µL hydrochloric acid

Solution 3: 50mmol phosphate, pH=7

  • 0.710g phosphate
  • 100mL distilled water
  • 2050µL hydrochloric acid

Solution 4: 50mmol tris, pH=9

  • 0.606g tris
  • 100mL distilled water
  • 750µL hydrochloric acid

Solution 5: 50mmol acetate and 100mmol sodium chloride, pH=5

  • 0.680g acetate
  • 0.584g sodium chloride
  • 100mL distilled water
  • 1050µL hydrochloric acid

Solution 6: 50mmol tris and 100mmol sodium chloride, pH=9

  • 0.606g tris
  • 0.584g sodium chloride
  • 100mL distilled water
  • 750µL hydrochloric acid


Molecular Weights (according to what was on the container)

  • acetate = 136.08g/mol
  • phosphate = 141.96g/mol
  • tris = 121.16g/mol


The quantum dots that were in solutions 1 and 4 seemed to fluoresce the most.