User:Nicole Bonan/Notebook/Quantum Dots/2014/01/31

From OpenWetWare
Jump to: navigation, search
BDLlogo notext lr.png Biomaterials Design Lab Report.pngMain project page
Next entryResultset next.png



Objective

Synthesize Mn-doped ZnS quantum dots using the synthesis described here

Description

Synthesis Protocol for Mn-doped ZnS nanocrystals with an Mn:Zn ratio of 1:7.5 and a final (Zn+Mn) concentration of 0.4mM

  1. Add 176μL of 5mM Zn(CH3COO)2 to a test tube
  2. Add 24μL of 5mM Mn((CH3COO)2) to the test tube
  3. Add 200μL of 40mM NH4CH3COO to the test tube
  4. Add 244μL of 0.55mg/mL protein to the test tube
  5. Add 250μL of 10mM NH4OH
    1. This should bring the pH up to 8.2. Check on this with a pH strip
  6. Bring the volume up to 2.5mL with water (Add 1606μL of water)
  7. Incubate at room temperature for 1 hour
  8. Add 200μL of 5mM Na2S
    1. When you add this add it drop wise (as best as you can) while vigorously shaking the test tube.
  9. Incubate at 37C for 5 days.

Data

Stock Solutions

All solutions are made in 250mL volumetric flasks.

  1. 5mM Zn(CH3COO)2
    1. FW = 219.49 g/mol
    2. V = 250mL
    3. mass to be measured = 0.274g
    4. actual mass = 0.274g
    5. actual concentration = 5mM
  2. 5mM Mn(CH3COO)2
    1. FW = 173.02 g/mol
    2. V = 250mL
    3. mass to be measured = 0.216g
    4. actual mass = 0.220 g (slightly pinkish hue)
    5. actual concentration = 5.1mM
  3. 40mM NH4(CH3COO)2
    1. FW = 77.08 g/mol
    2. V = 250mL
    3. mass to be measured = 0.7708g
    4. actual mass = 0.780g
    5. actual concentration = 40.5mM
  4. 10mM NH4OH
    1. initial M = 14.5M
    2. final V = 250mL
    3. volume to be diluted = 172μL
    4. actual concentration =
  5. 5mM Na2S
    1. FW = 240.18 g/mol
    2. V = 250mL
    3. mass to be measured = 0.300g
    4. actual mass = 0.300g
    5. actual concentration = 5.00mM

Notes

First solution synthesized, labeled "NB 176µL Zn soln", contained:

  • 176µL Zn(CH3COO)2
  • 24µL Mn(CH3COO)2
  • 200µL NH4CH3COO
  • 244µL protein
  • 330µL NH4OH

Note: When adding the Na2S, the pipette tip was dropped into the test tube. An unknown quantity of Na2S was added to the solution.


Second solution synthesized, labeled "NB 150µL Zn soln", contained:

  • 150µL Zn(CH3COO)2
  • 50µL Mn(CH3COO)2
  • 200µL NH4CH3COO
  • 244µL protein
  • 350µL NH4OH