User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/30

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June 30th, 2010

I restricted plasmids I extracted yesterday in order to ligate, the restrictions were the following ones:
LuxAB --- XbaI, PstI
Plasmid 18 --- XbaI, PstI
Plasmid J61002 --- SpeI, PstI
And I incubated restrictions to 37°C during 4h, then I dephosphated the plasmids

5μL LuxAB
2μL Plasmid J61002 (in the case of plasmid 18 I used 5μL because it was very diluted)
2μL Antarctic Phosphatese Buffer
1μL Antarctic Phosphatase
H2O
Total 20μL.

And I incubate restrictions overnight at 16°C.