User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/27

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June 27th, 2010

I made two ligations and I also ligated two controls:
luxAB --- ligated --- plasmid 17
luxAB --- ligated --- plasmid J61002 carrying ampicillin resistence (with constitutive promoter J23106)

Just plasmid 17
Just plasmid J61002

I followed the next protocol for ligation:
5μL luxAB (insert)
2μL vector
2μL Ligase Buffer 10x
1μL T4 ligase
10μL H2O
And I incubated during 19h to 16°C