User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/25

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June 25th, 2010

I dephosphated the two vectors I'm gonna use: plasmid J61002 carrying constitutive promoter J23106 and ampicillin resitence and plasmid 17. I followed the next protocol to dephosphate the two plasmids:
15μL DNA
3μL Buffer
1μL phosphatase
11μL H2O
30μL TOTAL
Then I made an agarose gel in order to make the ligation

LuxAB ----- Plasm 17 ----- Plasm J61002