User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/21

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June 21th, 2010

I searched some information about growing Vibrio fischerii and bioluminescence assays and I found some protocols: In an article about a toxicity assay I found they grew Vibrio fischerii DSM 7151 in two ekinds if media, LB and LM (luminescence medium); They used LB and LM media containing Artifitial Sea Water, they also used LB medium with 0.5M of NaCl to grow Vibrio fischerii but V.fischerii had a lower luminescence in that medium.

They grew V.fischerii in the following conditions:
pH 7
They used a rotary shaker (120rpm) at 30°C
(Their media contained a glicerol concentration at 3g/l because within this condition V.fischerii reaches its higher luminescence)

They showed also that in LB medium V.fischerii reaches its higher luminescence at OD650 of 3.7
In LM medium the highest luminescence its reached at OD650 of 2.6
(In the article are written the protocols of each medium)

Reference
Journal of Health Science,52(1) 9-16 (2006)
Analyses of Factors to Affect the Bioassay System Using Luminescent Bacterium Vibrio fischerii
Shinichi Nagata and Xiaojian Zhou
Environmental Biochemistry Group, Research Center for Inland Seas, Kobe University, 5-1-1 Fukaeminami, Higashinada, Kobe 658-0022, Japan.

In another article they grew V.fischerii in Sodium Chloride Complete Medium.
Reference
Proc. NatI. Acad. Sci. USA
Vol. 84, pp. 8912-8916, December 1987
Biochemistry
Yellow light emission of Vibriofischeri strain Y-1: Purification and
characterization of the energy-accepting yellow fluorescent protein*
(luciferase/bioluminescence/energy transfer)
S. COLETTE DAUBNER, ADRIENNE M. ASTORGA, GARY B. LEISMANt, AND THOMAS 0. BALDWINt
Department of Biochemistry and Biophysics, Texas A&M University, and Texas Agricultural Experiment Station, College Station, TX 77843