User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/14/2010/06/16
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June 16th, 2010
I make an agarose gel to 0.8% to prove if the PCR was correct; the gel showed no products, it could be due to a problem with the reactants (maybe Taq Polymerase), or due to a problem with the primer reverse; I made a mistake while designing the primers because I designed them for another strain of vibrio fischeri ATCC 7744 while the strain we used to obtain genomic DNA is MJ11; there is 4 mismatches in reverse primer.