User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/06/14/2010/06/15
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June 15th, 2010
While looking the items we received from Edimburgh we found that some primers, those for luxCDE, reverse and forward, were supposedly for LuxCDE from Photorhabdus luminescence but actually the primers were designed for LuxCDE from Photobacterium phosphoreum.
Then I made a PCR to get LuxAB from genomic DNA of Vibrio fischeri MJ11 following the next procedure: