User:Nelson Augusto Berrocal/Notebook/WiFi Coli 2010 Wet Lab/2010/05/06

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May 06th, 2010

I am going to make a double restriction with EcoRI and XbaI in order to open the plasmid pSB1AK3 (containing a doble terminator). We need the DNA more contcentrated.
20μL DNA
4μL Buffer
1μL BSA
2μL each enzyme
H2O
Total : 40μL
And I let the restriction sample incubating from 6h44pm and 37°C.