April 28th, 2010
I made two restrictions of the plasmid I extracted yesterday with EcoRI and PstI.
I followed the next protocol:
4μL Buffer 3
1μL each enzyme
After incubate 5h30 I made an agarose gel to 8% with the restricted plasmids and the original ones.
Ladder | DNA restricted 4μL | Plasmid 5μL | Ladder
0 1 2 3 4 5 6 7 8 9 <---Lanes
It seems that this gel it's OK, it showed two bands of 3kb in the lanes from 1 to 4.
(The small piece of 100bp that we expected didn't appear but we expect it to appear by making a PCR)