User:Nancy T. Miles/Notebook/N.Miles Lab 2013-09-03/2013/11/05

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Objective

Analysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs.

Description

  1. Before any measurement, take note of physical appearance of your samples (taking pics with cell phones helps here)
  2. Atomic Absorption
    1. centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.
    2. make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
    3. Take measurements of your standards and samples
    4. Make a graph showing concentration of Au vs Au:protein ratio

Data

Atomic Absorption

20131105 AAGoldStd ntm.png 20131105 AAHRP ntm.png 20131105 lyso ntm.png 20131105 myo ntm.png 20131105 myozoom ntm.png

UV-Vis

20131105 uvlyso1 ntm.png 20131105 uvlyso2 ntm.png 20131105 uvmyo1 ntm.png 20131105 uvmyo2 ntm.png