User:Nancy T. Miles/Notebook/N.Miles Lab 2013-09-03/2013/10/23

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Objective

To measure the activity of pepsin with pepsin in solution and pepsin-AuNPs in solution

Methods

The Pepsin-AuNPs made in the previous lab were used to measure the activity of pepsin

The ratios of Au:Pepsin that were used are as follows: 30, 50, 70, 90, 110, 130, 150, 170, 190, 210

HRP-AuNPs solutions were made with the following dilutions

  • 230
  • 250
  • 270
  • 290
  • 310
  • 330
  • 350
  • 370
  • 390
  • 410
  • Water up to 5mL

Myoglobin-AuNPs solutions were made with the following dilutions

  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
  • Water up to 5mL

Data

20131023 uvvis ntm.png