User:Nancy T. Miles/Notebook/N.Miles Lab 2013-09-03/2013/10/16
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The enzymatic activity of HRP-AuNPs will be observed by the conversion of luminol with H2O2.
Two different solutions were prepared:
100 uL of the HRP-AuNPs (with [Au]=0.25 mM and [HRP]=4.167uM) prepared from previous lab were used to test the kinetics of the reaction of luminol conversion with H2O2.
Remaking BSA-AuNPs from 8/28/2013
Procedures were obtained from User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/08/28
Transfer solution to a test tube and cap with aluminum foil and heated in the oven at 80C
Incredibly slow reaction was observed in this reaction of luminol with HRP-AuNPs and H2O2.