User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/02/26

Biomaterials Design Lab

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Objective Make 5 sets of PLA scaffolds: untreated PLA scaffolds, nanofiber-coated scaffolds by Brute-force method, biomineralization method of nanofiber formation with scaffolds, polydopamine-coated scaffolds, and polydopamine nanofiber-coated scaffolds Collect result data from last lab Methods Each set consisted of 3 test tubes, each containing 4 stacked PLA scaffolds. All scaffolds were Autoclaved at then end of each procedure. Plain scaffold The scaffolds were submerged in water Brute Force method 100:1 Au:Myoglobin nanofibers were made based on previous biomineralization protocol The nanofibers were then place onto the scaffolds and allowed to dry The dry nanofiber-coated scaffolds were then submerged in water Old method + scaffolds 100:1 Au:Myoglobin nanofibers were made following previously used protocol, but with the PLA scaffolds in the test tubes Polydopamine-coated scaffolds Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours Rinse with deionized water and allow scaffold to dry overnight Scaffolds submerged in water Polydopamine Nanofiber-coated scaffolds Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours Rinse with deionized water and allow scaffold to dry overnight Scaffolds submerged in water Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with polydopamine-coated scaffolds in solution Results Ag:BSA Nanofibers: Results from 02/25/2014 Set 1 (All ratios, room temperature) <br.> Set 2 (All ratios, heated to 80C) <br.> Set 3 (All ratios plus 1uL Au, heated to 80C) <br.>