User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/02/26

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Objective

  • Make 5 sets of PLA scaffolds: untreated PLA scaffolds, nanofiber-coated scaffolds by Brute-force method, biomineralization method of nanofiber formation with scaffolds, polydopamine-coated scaffolds, and polydopamine nanofiber-coated scaffolds
  • Collect result data from last lab

Methods

  • Each set consisted of 3 test tubes, each containing 4 stacked PLA scaffolds.
  • All scaffolds were Autoclaved at then end of each procedure.

Plain scaffold

  • The scaffolds were submerged in water

Brute Force method

  • 100:1 Au:Myoglobin nanofibers were made based on previous biomineralization protocol
  • The nanofibers were then place onto the scaffolds and allowed to dry
  • The dry nanofiber-coated scaffolds were then submerged in water

Old method + scaffolds

  • 100:1 Au:Myoglobin nanofibers were made following previously used protocol, but with the PLA scaffolds in the test tubes

Polydopamine-coated scaffolds

  • Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour
  • Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours
  • Rinse with deionized water and allow scaffold to dry overnight
  • Scaffolds submerged in water

Polydopamine Nanofiber-coated scaffolds

  • Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour
  • Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours
  • Rinse with deionized water and allow scaffold to dry overnight
  • Scaffolds submerged in water
  • Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with polydopamine-coated scaffolds in solution

Results

Ag:BSA Nanofibers: Results from 02/25/2014

  • Set 1 (All ratios, room temperature)

30 original.jpg 70 original.jpg<br.> 110 original.jpg 150 original.jpg

  • Set 2 (All ratios, heated to 80C)

30 oven.jpg 70 oven.jpg<br.> 110 oven.jpg 150 oven.jpg

  • Set 3 (All ratios plus 1uL Au, heated to 80C)

30 Au oven.jpg 70 Au oven.jpg<br.> 110 Au oven.jpg 150 Au oven.jpg