User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/02/11

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Results from Retention tests and new nanofibers

Objective

  • The results from the retention tests were recorded. The nanofiber-scaffolds were dried, placed in ethanol, PBS, or Strong Acid. Other nanofiber-scaffolds were placed in plain water, heated water, or in plain water which was later sonicated with the scaffold.
  • New nanofibers were made

Methods

Nanofiber preparations

Au stock solution:

  • 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM

Myoglobin stock solution:

  • 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM

BSA stock solution:

  • x (1 mol/66500g) x 0.010L =

Volumes of Au, protein, and water for each test tube:

  • Final [Au]=0.5mM, 5mL total volume

Results

  • Dried nanofiber scaffolds

Dried myo.jpg Dried myo 10x2.jpg

  • Ethanol

Ethanol myo.jpg Ethanol myo 10x2.jpg

  • Water heated

H2O heated myo.jpg H2O heated myo 10x.jpg

  • Plain Water

H2O myo.jpg H2O myo 10x2.jpg

  • Sonicated water

H2O son myo2.jpg H2O son myo2 10x.jpg

  • PBS

PBS myo2.jpg PBS myo 10x1.jpg

  • Strong Acid

SAcid myo.jpg SAcid myo 10x.jpg