User:Nancy T. Miles/Notebook/CHEM 572 Spr 2014/2014/02/04

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Observations from Jan. 28 Procedure

Objective

  • To obtain results (photos) from Jan. 28 procedure in which 12 test tubes were prepared for the formation of nanofibers, 3 of the test tubes containing PLA scaffolding.
  • The Gold:Myoglobin nanofibers were formed with a 100:1 ratio

Methods

  • Images of the nanofiber formation were taken
  • 7 More PLA scaffolds were created, but with taller dimensions than previous (instead of 12 layers, the new scaffolds will have ~50 layers).
  • Procedure from Jan. 28 will be repeated with new scaffolds
  • "Brute force" experiment begins
    • Of the nine nanofiber solutions made on Jan. 28, combine with a short scaffold (these scaffolds will be 1/3 the height of the original scaffolds; hence, these will be 4 layers tall).
    • Let these nanofiber&scaffold combination in solution dry overnight
    • Results will be analyzed next lab period.

Results

  • Nanofibers without scaffold

Nanofiber.jpg

  • Nanofiber&12-layer scaffold interaction:

Nanofiber-scaffold1.jpg Nanofiber-scaffold3.jpg

  • Nanofiber and scaffold interaction under microscope

PLA Fiber 2.5 2.bmp PLA fiber 10 1.bmp PLA Fibers 2.5 1.bmp

Sample Preparation

Au stock solution:

  • 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM

Myoglobin stock solution:

  • 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM

Volumes of Au, Myoglobin, and water for each test tube:

  • Final [Au]=0.5mM, 5mL total volume