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Objective
- Test the effects of various Gold concentrations on the formation of purple solution or fibers. The concentration of BSA is kept constant.
- Continue the protein expression from experiment on November 15th.
Description
- Synthesis of Conjugated Gold Nanoparticles
1. Four Test tubes were prepared.
- Test Tube 1 contained 0.1ml BSA (30µM), 0.2ml HAuCl4(4.17mM), and 0.7ml H2O.
- Test Tube 2 contained 0.1ml BSA (30µM), 0.143ml HAuCl4 (4.17mM, and 0.757ml H2O.
- Test Tube 3 contained 0.1ml BSA (30µM), 0.115ml HAuCl4 (4.17mM), and 0.785ml H2O.
- Test Tube 4A contained 0.1ml BSA (30µM) and excess of HAuCl4. Solution was filled to 1ml with H2O.
- Test Tube 4B contained 0.1ml BSA (30µM), 0.086ml HAuCl4 (4.17mM) and 0.814ml water.
2. The four tubes were placed in a heatblock set at 80°C.
- To each medium prepared yesterday, 1.1ml of Ampicillin (300mg Ampicilin + 4ml water), 1ml of cell suspension (which grew up overnight) and 1ml of (380mg Isopropyl β-D-1-thiogalactopyranoside in 4ml H2O) were added.
- Placed the four mixtures in the shaker for several hours to facilitate cell proliferation
- The four mixtures were centrifuged.
- Medium was added to the cells and the mixture was placed into fridge set at -80°C.
Data
Tube 1: Clear solution with yellow fibers.
Tube 2: Clear solution with purple fibers.
Tube 3: Homogeneous purple solution.
Tube 4A: Homogeneous yellow solution.
Tube 4B: Clear solution with yellow fibers.
Notes
This area is for any observations or conclusions that you would like to note.
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