User:Nader A. Khamis/Notebook/Experimental Biological Chemistry AU Fall 2011/2011/09/28

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  • Repeat experiment performed on September 27th using H2O as buffer.
  • Check for the presence of cells that were plated on September 27th.


  1. Approximately 0.581 ml HAuCl4 (0.25mM), 0.974ml BSA (15uM), and 8.45ml H2O Buffer were pipetted into a test tube.
  2. The tube was placed in the oven for 2 hours and was not removed.
  3. Every 30 minutes, a UV-Vis Spectra was obtained using a Shimadzu 2550 spectrometer.
  4. Following 2 hours, the test tube was removed, wrapped in aluminum foil and was stored at room temperature.


  • The graph below is the UV-Vis Spectra of HAuCl4/BSA/H2O obtained in the wavelength range of 200-800nm. This graph was zoomed in at 550nm, which is why the range is from 400nm to 800nm.

Experimental - Openwetware - September 28th 2011.jpg

  • The graph below exhibits the change in absorbance at 550nm over a period of 2 hours

Experimental - Openwetware - 2-September 28th 2011.jpg

  • Observation showed that the solution was purple and contained no fibers.



1. Conjugated Gold Nanoparticle Synthesis

  • According to the two graphs, the absorbance values of the solution increased without fluctuation.
  • Observations showed that a clear purple solution formed. No fibers appeared.
  • This indicated that conjugated gold nanoparticles formed.

2. The plates contained no cells.

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