User:NYOUSHA Yousefi/Notebook/Protein Engineering/2012/09/25

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Entry title

Protoporphyrin IX and Zinc acetate, day 2


The Procedure performed on day 1 was repeated with slight changes


  1. 10 ml of of DMF was added to a 50ml round bottom flask and was brought to boiling point on a heating mantle and under a condenser.
  2. After it started boiling, 0.1006g of protoporphyrin ix was added to the boiling DMF and it was allowed to completely dissolve for 20min while still boiling.
  3. After 20min, 0.0230g of zinc acetate was added to the solution and it was heated for 30min to allow the reaction take place.
  4. Then the flask was cooled in ice bath for 15min.
  5. 100ml of cooled distilled water was added to the solution.
  6. A Filter paper was measured before filtration, it weighed 0.0706g.
  7. The solution was filtered with a different filter paper with much smaller filter pores.
  8. The precipitate and the filter paper were place on a plate and were raped by aluminum paper to keep it away from light.
  9. The filter paper and the precipitant were allowed to completely dry out to be ready for final mass measurements.