User:Morgan L. Paull/Notebook/Genetic Fence/2011/01/20

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Checking Act2LacO and parts which contain it

  • Cut 10 parts, either Act2LacO or parts which contain Act2LacO, with Eco and Pst in 10μL reactions
  • Each gel image is separated by more time running on gel - the first after 15, the second after another 15, the final after another 30 minutes


Number: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Name: KB+ Ladder KB+ Ladder
Expected Length(bp): 273 273 600+ 600+ 1119 438 438 714 714 1446 1446 1119 93 93 2657 2657
Correct Size: yes + yes + yes + yes + yes + yes yes + yes yes short yes blank not visible + not visible + no band visible blank

File:Act2laccheck1 1-20-11.tif File:Act2laccheck2 1-20-11.tif File:Act2laccheck3 1-20-11.tif


Gel Extraction

  • Cut out the proper length band (see final gel image)
  • Performed gel extraction in preparation to ligate the Act2LacO promoter into a V0120 backbone

Team Fence Boxes Clean-up

  • Culled 5 team fence boxes from the -20°C freezer, consolidating into just three boxes - hopefully at some point only 2.

2011-01-20 16-56-46 531.jpg

Transformations

  • Transformed minipreps from several fence parts to make glycerol stocks of them