User:Morgan L. Paull/Notebook/Genetic Fence/2011/01/17

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Diagnostic Digest of GalUAS35s parts Cut the two tubes I have each with xba pst and eco pst. The two tubes were an unlabeled one and one labeled as a ligation mix. All reactions were 10μL, with 1μL FD Green buffer Used 7μL of the gel extracted V0120 Backbone from yesterday, and 1μL of the 35s annealed oligos, at the concentrations listed below 1 2 3 4 5 6 7 8 9 10 11 12 13 KB+ Ladder 5xGalUAS (unlabeled) Eco Pst 5xGalUAS (ligation) Eco Pst 5xGalUAS (unlabeled) Xba Pst 5xGalUAS (ligation) Xba Pst Ligation mix plasmid Unlabeled plasmid KB+ Ligating 35s.A.2 anneal into V0120 1x insert concentration 1μL 35s 7μL BB 2μL #3 Roche buffer 10μL #4 Roche buffer 1μL #5 Roche buffer 1/10x insert concentration 1μL 1/10 35s 7μL BB 2μL #3 Roche buffer 10μL #4 Roche buffer 1μL #5 Roche buffer 1/100x insert concentration 1μL 1/100 35s 7μL BB 2μL #3 Roche buffer 10μL #4 Roche buffer 1μL #5 Roche buffer 1/1000x insert concentration 1μL 1/1000 35s 7μL BB 2μL #3 Roche buffer 10μL #4 Roche buffer 1μL #5 Roche buffer Control 1μL DH2O 7μL BB 2μL #3 Roche buffer 10μL #4 Roche buffer 1μL #5 Roche buffer