User:Morgan L. Paull/Notebook/Genetic Fence/2011/01/13

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Gibson overhang PCR attempt 1

  • Ran the PCR from tuesday on a 1% agarose gel
  • 1μL PCR product
  • 2μL Loading dye
  • 9μL DH2O
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KB+ Ladder M1F1 M2F2 M3F3 M4F4 M5F5 M6F6 M7F3 M9F9 M10F3 KB+ Ladder
Expected Length(bp): 320 730 300 540 480 1000 300 500 300

File:Gibson pcr one 1 11.tif

Checking plasmids from tuesday's gibson PCR

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KB+ Ladder F1 - VP16 F2 - RXRLc F3 - Nost-stop F4 - Ent cup F5 - Gal4 F6 - EcR F9 - Barstar KB+ Ladder
Expected Length(bp): 5246 5659 5226 5473 5408 4078 5243

File:Plasmids alone check gibson pcr1 1 13.tif